Multiple magnesium ions in the ribonuclease P reaction mechanism.

  title={Multiple magnesium ions in the ribonuclease P reaction mechanism.},
  author={D H Smith and Norman R. Pace},
  volume={32 20},
The ribozyme ribonuclease (RNase) P cleaves precursor transcripts to produce the mature 5'-end of tRNAs. This hydrolysis reaction has a divalent cation requirement that is primarily catalytic, rather than structural; RNase P can be considered a metalloenzyme. Kinetic analysis shows that the RNase P catalytic mechanism has a cooperative dependence upon Mg2+ concentration. At least three Mg2+ ions are required for optimal activity, suggesting a multiple metal ion mechanism. The 2'-OH at the site… CONTINUE READING


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