Multiple immunoenzyme labeling using heat treatment combined with the polymer method: an analysis of the appropriate inactivation conditions of primary antibodies.

@article{Ikeda2011MultipleIL,
  title={Multiple immunoenzyme labeling using heat treatment combined with the polymer method: an analysis of the appropriate inactivation conditions of primary antibodies.},
  author={Katsuhide Ikeda and Takao K Suzuki and Genshu Tate and Toshiyuki Mitsuya},
  journal={Acta histochemica},
  year={2011},
  volume={113 2},
  pages={117-24}
}
Multiple immunoenzyme labeling is of considerable value to detect several antigens in the same specimen, although this technique is limited when the primary antibodies have been raised in the same animal species. Multiple immunoenzyme labeling using heat treatment is a simple, reliable and straightforward technique wherein the heat treatment prevents mixed… CONTINUE READING