Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals.

@article{Mukherjee2007MultipleGM,
  title={Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals.},
  author={Falguni Mukherjee and Jainendra Jain and Vipul Patel and Mrinalini Nair},
  journal={Journal of medical microbiology},
  year={2007},
  volume={56 Pt 10},
  pages={1309-16}
}
Brucella-specific nucleotide sequences encoding the BCSP 31 kDa protein, Omp2 and the 16S rRNA were employed in three independent diagnostic PCR assays. Results of the three PCR assays on six reference strains of Brucella were in complete agreement. The results of PCR assays based on bcsp and omp2 on 19 Indian field isolates (human, bovine and murine tissues) also agreed completely. However, when the 16S rRNA gene was employed as the diagnostic target in the PCR, only 14 out of these 19… CONTINUE READING

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