Multiple forms of cytochrome P-450 in rabbit colon microsomes.

  title={Multiple forms of cytochrome P-450 in rabbit colon microsomes.},
  author={Mitsuo Kaku and Emi Kusunose and Shozo Yamamoto and Kosuke Ichihara and M. Kusunose},
  journal={Journal of biochemistry},
  volume={97 2},
Three cytochrome P-450 preparations, designated as cytochrome P-450ca, cytochrome P-450cb, and cytochrome P-448c fraction, were separated and purified about 23-, 50-, and 29-fold, respectively, from the cholate extracts of rabbit colon mucosa microsomes. Their specific contents were 1.2, 2.6, and 1.5 nmol of cytochrome P-450 per mg of protein, respectively. Cytochrome P-450ca and cytochrome P-450cb migrated as heme-containing polypeptide bands with molecular weights of about 53,000 and 57,000… CONTINUE READING