Multiple forms of affinity-labeled estrogen receptors in rat distinct pituitary cells.

@article{GeffroyRoisn1992MultipleFO,
  title={Multiple forms of affinity-labeled estrogen receptors in rat distinct pituitary cells.},
  author={S Geffroy-Roisn{\'e} and J. Roman Duval and M. L. Thieulant},
  journal={Endocrinology},
  year={1992},
  volume={131 3},
  pages={1503-10}
}
The presence of multiple monomeric forms has been described for estrogen receptor (ER) in different target tissues. Using [3H]tamoxifen aziridine ([3H]TA) to covalently label ER and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to analyze labeled products, ER forms were investigated in pituitary cytosol and purified nuclei from male rats. ER forms were also compared in cellular extracts from gonadotrope-enriched populations (GP), prepared using the fast method of centrifugal… CONTINUE READING