Multiple bcl-2/Ig gene rearrangements in persistent polyclonal B-cell lymphocytosis.


Persistent polyclonal B-cell lymphocytosis is a benign lymphoproliferative disorder of unknown aetiology occurring exclusively in women, characterized by typical binucleated lymphocytes, polyclonal expansion of B cells and elevated serum IgM. Owing to the role of Bcl-2 oncogene in inhibition of apoptosis, we have investigated the presence of the bcl-2/Ig gene rearrangement. Bcl-2/Ig gene rearrangement was determined by polymerase chain reaction targeting the usual breakpoint regions of the t(14;18). Bcl-2/Ig gene rearrangement was identified in all six patients and, more importantly, multiple rearrangements were present in five patients. The frequency of the bcl-2/Ig gene rearrangement is estimated to be of one translocation in 1 x 10(2) to 1 x 10(3) peripheral blood mononuclear cells. We conclude that persistent polyclonal B-cell lymphocytosis is associated with bcl-2/Ig gene rearrangement. These findings are of clinical importance because these patients may be misdiagnosed as having a leukaemic expression of non-Hodgkin's lymphoma.


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@article{Delage1997MultipleBG, title={Multiple bcl-2/Ig gene rearrangements in persistent polyclonal B-cell lymphocytosis.}, author={Robert Delage and J Roy and Lesley Jacques and Vincent Bernier and J. M. Del{\^a}ge and Andr{\'e} Darveau}, journal={British journal of haematology}, year={1997}, volume={97 3}, pages={589-95} }