Multiple activation of chloroform in hepatic microsomes from uninduced B6C3F1 mice.


The covalent binding of 14C-label to proteins and lipids was measured after incubation of hepatic microsomes from B6C3F1 mice with different concentrations of [14C]chloroform and oxygen. The effect of reduced glutathione on the covalent binding curves was also investigated. The results indicated that chloroform is activated through three processes: the… (More)