Multiple Response Optimization of a HPLC Method for the Determination of Enantiomeric Purity of S-Ofloxacin

  title={Multiple Response Optimization of a HPLC Method for the Determination of Enantiomeric Purity of S-Ofloxacin},
  author={Valliappan Kannappan and Sai Sandeep Mannemala},
The aim of the study was to develop a new HPLC method for direct chiral separation of Ofloxacin enantiomers using polar non-aqueous mobile phase by application of response surface methodology. Rotatable central composite design (CCD) with eight factorial points, six axial points and six replications in central point was used to evaluate the influence of three independent variables (concentration of methanol, diethylamine and flow rate) on the output responses (capacity factor of first peak… Expand

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