Multiple DNA extractions coupled with stable-isotope probing of anthracene-degrading bacteria in contaminated soil.

@article{Jones2011MultipleDE,
  title={Multiple DNA extractions coupled with stable-isotope probing of anthracene-degrading bacteria in contaminated soil.},
  author={Maiysha D. Jones and David R Singleton and Wei Sun and Michael D Aitken},
  journal={Applied and environmental microbiology},
  year={2011},
  volume={77 9},
  pages={2984-91}
}
In many of the DNA-based stable-isotope probing (SIP) studies published to date in which soil communities were investigated, a single DNA extraction was performed on the soil sample, usually using a commercial DNA extraction kit, prior to recovering the (13)C-labeled (heavy) DNA by density-gradient ultracentrifugation. Recent evidence suggests, however, that a single extraction of a soil sample may not lead to representative recovery of DNA from all of the organisms in the sample. To determine… CONTINUE READING

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