Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging.

@article{Centonze1998MultiphotonEP,
  title={Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging.},
  author={Victoria E Centonze and Jackie G. White},
  journal={Biophysical journal},
  year={1998},
  volume={75 4},
  pages={2015-24}
}
Multiphoton excitation fluorescence imaging generates an optical section of sample by restricting fluorophore excitation to the plane of focus. High photon densities, achieved only in the focal volume of the objective, are sufficient to excite the fluorescent probe molecules by density-dependent, multiphoton excitation processes. We present comparisons of confocal with multiphoton excitation imaging of identical optical sections within a sample. These side-by-side comparisons of imaging modes… CONTINUE READING

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