Multiparametric in situ messenger RNA hybridization analysis to detect metastasis-related genes in surgical specimens of human colon carcinomas.

@article{Kitadai1995MultiparametricIS,
  title={Multiparametric in situ messenger RNA hybridization analysis to detect metastasis-related genes in surgical specimens of human colon carcinomas.},
  author={Yasuhiko Kitadai and Lee M. Ellis and Yoko Takahashi and Corazon D. Bucana and Hiroko Anzai and Eiichi Tahara and Isaiah Josh Fidler},
  journal={Clinical cancer research : an official journal of the American Association for Cancer Research},
  year={1995},
  volume={1 10},
  pages={1095-102}
}
We examined the expression of several genes that regulate different steps of metastasis in surgical specimens of human colon carcinomas. The expression of epidermal growth factor receptor (growth), basic fibroblast growth factor [(bFGF), angiogenesis], type IV collagenase (invasion), E-cadherin (adhesion), and multidrug-resistant (mdr)-1 (drug resistance) mRNA was examined using an in situ mRNA hybridization (ISH) technique and Northern blot analysis. Dukes' stage C and D tumors exhibited a… CONTINUE READING