Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression

@inproceedings{Fath2011MultiparameterRA,
  title={Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expression},
  author={Stephan Fath and Asli Petra Bauer and Michael Liss and Anne Spriestersbach and Barbara Maertens and Peter Hahn and Christine Ludwig and Frank Sch{\"a}fer and Marcus Graf and Ralf Wagner},
  booktitle={PloS one},
  year={2011}
}
Autologous expression of recombinant human proteins in human cells for biomedical research and product development is often hampered by low expression yields limiting subsequent structural and functional analyses. Following RNA and codon optimization, 50 candidate genes representing five classes of human proteins--transcription factors, ribosomal and polymerase subunits, protein kinases, membrane proteins and immunomodulators--all showed reliable, and 86% even elevated expression. Analysis of… CONTINUE READING
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