Multimode gradient high performance liquid chromatography mass spectrometry method applicable to metabolomics and environmental monitoring.

@article{Ammann2016MultimodeGH,
  title={Multimode gradient high performance liquid chromatography mass spectrometry method applicable to metabolomics and environmental monitoring.},
  author={Adrian A. Ammann and Marc J-F Suter},
  journal={Journal of chromatography. A},
  year={2016},
  volume={1456},
  pages={145-51}
}
Metabolomics or environmental investigations generate samples containing very large numbers of small molecular weight analytes. A single mode chromatographic separation excludes a substantial part of such complex analyte mixtures. For instance, a reversed-phase separation would not retain ionic species, resulting in a correspondingly huge front peak. To address this problem, we used two commercially available mixed-mode ion-exchange reversed-phase columns (WAX-1 and WCX-1) in sequence in a… CONTINUE READING