Multimode fibre: Light-sheet microscopy at the tip of a needle

  title={Multimode fibre: Light-sheet microscopy at the tip of a needle},
  author={Martin Pl{\"o}schner and Věra Koll{\'a}rov{\'a} and Zbyněk Do{\vs}t{\'a}l and Jonathan Nylk and Thomas B Barton-Owen and David E. K. Ferrier and Radim Chmel{\'i}k and Kishan Dholakia and Tom{\'a}{\vs} {\vC}i{\vz}m{\'a}r},
  journal={Scientific Reports},
Light-sheet fluorescence microscopy has emerged as a powerful platform for 3-D volumetric imaging in the life sciences. Here, we introduce an important step towards its use deep inside biological tissue. Our new technique, based on digital holography, enables delivery of the light-sheet through a multimode optical fibre – an optical element with extremely small footprint, yet permitting complex control of light transport processes within. We show that this approach supports some of the most… 
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Microfabricated devices for single objective single plane illumination microscopy (SoSPIM).
The suitability of the replicated polymer based sample holder for single-lens light sheet microscopy was finally demonstrated by imaging breast cancer spheroids and the optical quality of inexpensive replicas from both types of master molds was evaluated.
Fast volumetric fluorescence imaging with multimode fibers.
A compact multimode fiber endoscope which employs wavefront shaping with a digital micromirror device (DMD) and achieves accurate focusing at various depths in the sample through rapid switching of holographic patterns by the DMD is proposed.
Label-free CARS microscopy through a multimode fiber endoscope.
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Technical implementations of light sheet microscopy
The most advanced L SFM implementations are reviewed to assist new LSFM users in the choice of the LSFm set‐up that suits their needs best and also focuses on new commercial microscopes and “do‐it‐yourself” strategies.


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Images of fluorescent beads and neurons in mouse neocortex exhibited superior axial resolution and contrast in the miniSPIM-mode compared to images recorded in epi-illumination mode, and may enable novel in vivo imaging approaches.
Exploiting multimode waveguides for pure fibre-based imaging
A novel approach is presented by utilizing disordered light within a standard multimode optical fibre for lensless microscopy and optical mode conversion and showing how such control can realize a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices.
Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation.
The results reveal that light-sheets generated by pulsed near-infrared Bessel beams and two photon excitation provide the best image resolution, contrast at both a minimum amount of artifacts and signal degradation along the propagation of the beam into the sample.
A multi-mode fiber probe for holographic micromanipulation and microscopy.
It is demonstrated that the complex light propagator of a real multimode fiber can be directly measured and open the way towards the fabrication of endoscopic probes which could be capable of seeing and manipulating single cells deep into biological tissues.
Wavefront corrected light sheet microscopy in turbid media
Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations.
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A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.
Shaping the light transmission through a multimode optical fibre: complex transformation analysis and applications in biophotonics.
It is shown that using this approach one can generate an arbitrary output optical field within the accessible field of view and range of spatial frequencies given by fibre core diameter and numerical aperture, respectively, that contains over 80% of the total available power.
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.
Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection.
The benefits of combining light-sheet macroscopy and HiLo background rejection are demonstrated in optically cleared whole mouse brain samples, using both green fluorescent protein (GFP)-fluorescence and dark-field scattered light contrast.
Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination