Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene.

@article{Kim2004MultimodalityIO,
  title={Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene.},
  author={Young Jun Kim and Purnima Dubey and Pritha Ray and Sanjiv Sam Gambhir and Owen N Witte},
  journal={Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging},
  year={2004},
  volume={6 5},
  pages={331-40}
}
PURPOSE Previous work showed quantitative imaging of T-cell migration into a tumor site by positron emission tomography (PET), using retroviral transduction of mutated thymidine kinase (sr39TK) reporter genes into immunized T-lymphocytes. PROCEDURES AND RESULTS In order to improve the sensitivity and flexibility of the imaging analysis, lentivirus, that expressed sr39TK, was used to transduce the lymphocytes that migrated to an immunogenic sarcoma site. In comparison to retrovirally… CONTINUE READING