Multimodal control of transcription factor Pap1 in Schizosaccharomyces pombe under nitrosative stress.

  title={Multimodal control of transcription factor Pap1 in Schizosaccharomyces pombe under nitrosative stress.},
  author={P. Kar and Pranjal Biswas and Sanjay Ghosh},
  journal={Biochemical and biophysical research communications},
  volume={489 1},
Schizosaccharomyces pombe Pap1, a bZIP transcription factor, is highly homologous to the mammalian c-Jun protein that belongs to the AP1 family of transcriptional regulators. The role of transcription factor Pap1 has been extensively studied under oxidative stress. Two cysteine residues in Pap1p namely, C278 and C501 form disulfide linkage under oxidative stress resulting in nuclear accumulation. We first time showed the involvement of Pap1 in the protection against nitrosative stress. In the… Expand


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Spy1 plays protective roles against nitrosative and nutritional stress in the fission yeast and is transcriptionally up-regulated by nitrosatives and nutritional stresses in a Pap1-dependent manner. Expand
Nitrosative stress induces a novel intra-S checkpoint pathway in Schizosaccharomyces pombe involving phosphorylation of Cdc2 by Wee1.
A novel intra-S-phase checkpoint that is activated in S. pombe under nitrosative stress is discovered and it is found that Wee1 phosphorylates Cdc2 Tyr15 which leads to replication slowdown in the fission yeast under nitosative stress. Expand
Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress.
Genes of the pathway meiosis, cell cycle, spliceosome and oxidative phosphorylation were mostly affected under nitrosative stress in the fission yeast, and surprisingly, Pap1 regulated genes in S. pombe were distinctly different under nit ROSative stress. Expand
The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H2O2
It is shown that a subset of Pap1-dependent genes, such as those coding for the efflux pump Caf5, the ubiquitin-like protein Obr1 or the dehydrogenase SPCC663.08c, only require nuclear Pap1 for activation, whereas another subset of genes do need oxidized Pap1 to form a heterodimer with the constitutively nuclear transcription factor Prr1. Expand
A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
We describe a Pap1–Oxs1 pathway for diamide-induced disulfide stress in Schizosaccharomyces pombe, where the nucleocytoplasmic HMG protein Oxs1 acts cooperatively with Pap1 to regulate transcription.Expand
Regulation of cell cycle and stress responses under nitrosative stress in Schizosaccharomyces pombe.
A novel molecular mechanism of cell cycle control under nitrosative stress is proposed based on the experimental results and bioinformatics analysis of the fission yeast Schizosaccharomyces pombe. Expand
Effect of nitrosative stress on Schizosaccharomyces pombe: inactivation of glutathione reductase by peroxynitrite.
This is the first report of S-nitrosoglutathione (GSNO) reductase activity in S. pombe and its inactivation by GSNO and the inactivation of GR and glutathione peroxidase in the presence of various reactive nitrogen species in vivo. Expand
The pap1(+) gene of fission yeast is transcriptionally regulated by nitrosative and nutritional stress.
It is demonstrated that transcription of the Schizosaccharomyces pombe pap1(+) gene is positively regulated by nitrosative and nutritional stress in a Pap1p-dependent manner. Expand
Activation and regulation of the Spc1 stress-activated protein kinase in Schizosaccharomyces pombe
It is reported here that Spc1 is activated by multiple forms of stress, including high temperature and oxidative stress, and that of regulation may be commonly employed to modulate MAPK signal transduction pathways in eukaryotic species. Expand
The Glycolytic Metabolite Methylglyoxal Activates Pap1 and Sty1 Stress Responses in Schizosaccharomyces pombe*
It is shown that the two principal oxidative stress response pathways of Schizosaccharomyces pombe, Sty1 and Pap1, are involved in the response to methylglyoxal toxicity. Expand