Multimeric assembly and biochemical characterization of the Trax/Translin endonuclease complex

@inproceedings{Tian2011MultimericAA,
  title={Multimeric assembly and biochemical characterization of the Trax/Translin endonuclease complex},
  author={Yuan Tian and Dhirendra Kumar Simanshu and Manuel Ascano and Ruben Diaz-Avalos and Ah Young Park and Stefan Andreas Juranek and William E. Pine Kenneth H. Pollock Joseph E. Hightower Tho Rice and Qian Yin and Carol V. Robinson and Thomas Tuschl and Dinshaw J. Patel},
  booktitle={Nature Structural &Molecular Biology},
  year={2011}
}
Trax–translin heteromers, also known as C3PO, have been proposed to activate the RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand. We report on the crystal structure of hexameric Drosophila C3PO formed by truncated translin and Trax, along with electron microscopic and mass spectrometric studies on octameric C3PO formed by full-length translin and Trax. Our studies establish that Trax adopts the translin fold, possesses catalytic… CONTINUE READING