Multilocus sequence typing versus pulsed-field gel electrophoresis for characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolates.

@article{Nemoy2005MultilocusST,
  title={Multilocus sequence typing versus pulsed-field gel electrophoresis for characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolates.},
  author={Lucia L. Nemoy and Mamuka Kotetishvili and Justine T Tigno and Ananda Keefer-Norris and Anthony D. Harris and Eli N. Perencevich and Judith A. Johnson and Dave Torpey and Alexander Sulakvelidze and John G Morris and Oscar Colin Stine},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 4},
  pages={1776-81}
}
Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains are emerging pathogens. Molecular typing of ESBL-producing E. coli is useful for surveillance purposes, to monitor outbreaks and track nosocomial spread. Although pulsed-field gel electrophoresis (PFGE) is the current "gold standard" for bacterial molecular typing, multilocus sequence typing (MLST) may offer advantages. Forty ESBL-producing E. coli isolates were selected at random from a cohort of intensive care unit… CONTINUE READING

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