Multilocus sequence typing of oenological Saccharomyces cerevisiae strains.

  title={Multilocus sequence typing of oenological Saccharomyces cerevisiae strains.},
  author={Rosario Palacios Mu{\~n}oz and Alicia Estacio Gomez and Virginia Robles and Patricia L Rodr{\'i}guez and Eduardo Cebollero and Laura Tabera and Alfonso V. Carrascosa and Ram{\'o}n Hinojoza Gonz{\'a}lez},
  journal={Food microbiology},
  volume={26 8},
This study describes the application of a multilocus sequence typing (MLST) analysis for molecular discrimination at the strain level of Spanish wine yeast strains. The discrimination power of MLST is compared to mitochondrial RFLP analysis. Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. Ten polymorphic sites were found in the five loci analyzed showing 13 different genotypes, with 11 of them… CONTINUE READING

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