Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence

@article{Nadeau1992MultilocusMF,
  title={Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence},
  author={Joseph H. Nadeau and Hendrick G. Bedigian and Gayle Bouchard and T{\'e}r{\`e}se Denial and Michael R. Kosowsky and Robert Norberg and Sherri Pugh and Evelyn Sargeant and R. Turner and Beverly J. Paigen},
  journal={Mammalian Genome},
  year={1992},
  volume={3},
  pages={55-64}
}
Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a powerful marker system for genome analysis because each primer amplifies multiple products, and cloning, sequencing, and hybridization are not required. We have evaluated this typing system for the mouse by identifying optimal PCR conditions; characterizing effects of GC content, primer length, and multiplexed primers; demonstrating considerable variation among a panel of inbred strains; and… CONTINUE READING

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References

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Showing 1-10 of 26 references

Genetic maps of mouse Chromosome 17 including 12 new anonymous DNA loci and 25 anchor loci

  • J. H. Nadean, B. Herrmann, +12 authors D. Barlow
  • Genomics
  • 1991

Multilocus markers for genome analysis ucts to random-length higher molecular weight fragments

  • J. H. Nadeau
  • Nucl Acids Res
  • 1991
1 Excerpt

Multiplex PCR: Genomic fingerprinting with pairwise combinations of primers

  • J. Welsh, M. McClelland
  • Nucl Acids Res
  • 1991

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