Multilineage potential of adult human mesenchymal stem cells.

@article{Pittenger1999MultilineagePO,
  title={Multilineage potential of adult human mesenchymal stem cells.},
  author={Mark F. Pittenger and Alastair Morgan Mackay and S C Beck and Rama K. Jaiswal and R Douglas and Joseph D. Mosca and Mark A. Moorman and D Simonetti and Stewart Craig and Daniel Robert Marshak},
  journal={Science},
  year={1999},
  volume={284 5411},
  pages={
          143-7
        }
}
Human mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells were isolated from marrow aspirates of volunteer donors. These cells displayed a stable phenotype and remained as a monolayer in vitro… 
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28 – Mesenchymal Stem Cells

In vitro Differentiation Potential of Mesenchymal Stem Cells

This review focuses on the in vitro differentiation capability of cells derived from adult human tissue and the limitations and promises of these studies in the context of tissue engineering.

Multilineage stem cells in the adult

It is clear that purified perivascular cells exhibit multiple mesodermal developmental potentials and become indistinguishable from conventionally derived mesenchymal stem cells after in vitro culture, but the possible roles played by these blood vessel-bound cells in organogenesis and adult tissue repair remain elusive.

Pluripotency of mesenchymal stem cells derived from adult marrow

We report here that cells co-purifying with mesenchymal stem cells—termed here multipotent adult progenitor cells or MAPCs—differentiate, at the single cell level, not only into mesenchymal cells,…

Nonstimulated human uncommitted mesenchymal stem cells express cell markers of mesenchymal and neural lineages.

The depicted "molecular signature" of these early uncommitted progenitors, in the absence of differentiation stimuli, is consistent with their multipotentiality and plasticity as suggested by several in vitro and in vivo studies.

SSEA-4 identifies mesenchymal stem cells from bone marrow.

It is reported that SSEA-4, an early embryonic glycolipid antigen commonly used as a marker for undifferentiated pluripotent human embryonic stem cells and cleavage to blastocyst stage embryos, also identifies the adult mesenchymal stem cell population.

Multi-lineage potential of human mesenchymal stem cells following clonal expansion.

The data provide direct experimental evidence that cultures of bone marrow-derived mesenchymal cells contain individual cells that fulfil two essential stem cell criteria: (i) extensive self-renewal capacity and (ii) multi-lineage potential.

Plasticity of Bone Marrow–Derived Stem Cells

The literature that lends credibility to the theory that highly plastic BMCs have a role in maintenance and repair of nonhematopoietic tissue is reviewed and the possible mechanisms by which this may occur are discussed.

Circulating mesenchymal stem cells.

Enhanced stem cells characteristic of fibroblastic mesenchymal cells from HHT patients

HHT cells possesses the same surface markers expression of mesenchymal stem cells, they are highly clonogenic, showing a high proliferation potential and an enhanced capacity of self renewal.
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33 References

Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues

Marrow stromal cells present an intriguing model for examining the differentiation of stem cells and have several characteristics that make them potentially useful for cell and gene therapy.

Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro.

Embryonic stem cell lines derived from human blastocysts.

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate…

Mesenchymal Stem Cells

  • A. Caplan
  • Biology
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society
  • 1991
The study of mesenchymal stem cells, whether isolated from embryos or adults, provides the basis for the emergence of a new therapeutic technology of self‐cell repair.

Phenotypic and functional comparison of cultures of marrow‐derived mesenchymal stem cells (MSCs) and stromal cells

The morphology, phenotype, and in vitro function of cultures of MSCs and traditional marrow‐derived stromal cells (MDSCs) from the same marrow sample are examined to suggest that M SCs represent an important cellular component of the bone marrow microenvironment.

Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies.

The Chondrogenic Potential of Human Bone-Marrow-Derived Mesenchymal Progenitor Cells*

The successful induction of in vitro chondrogenesis with human bone-marrow-derived osteochondral progenitor cells is demonstrated in a reliable and reproducible culture system and provides evidence for the clinical utility of these cells in the repair of bone and cartilage.

Growth kinetics, self‐renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation

The use of population doubling potential as a measure of biological age suggests that MSCs are intermediately between embryonic and adult tissues, and as such, may provide an in situ source for mesenchymal progenitor cells throughout an adult's lifetime.

Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5‐azacytidine

Support is provided for the suggestion that mesenchymal stem cells in the bone marrow of postnatal organisms may provide a source for myoprogenitor cells which could function in clinically relevant myogenic regeneration.

Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers

The high proliferative potential of bone marrow FCFC and their ability to serve as common precursors of bone and cartilage‐forming cells makes them probable candidates for the role of osteogenic stem cells.