Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli.

@article{Leduc1989MultilayeredDO,
  title={Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli.},
  author={M T Leduc and Claude Fr{\'e}hel and Ekkehard Siegel and Jean van Heijenoort},
  journal={Journal of general microbiology},
  year={1989},
  volume={135 5},
  pages={1243-54}
}
When a staining technique using phosphotungstic acid (PTA) in 10% (w/v) chromic acid was applied to cells of Escherichia coli, the periplasmic space was seen as a dark 15-nm-thick layer of uniform appearance and constant width. Our observations are consistent with peptidoglycan being the main material stained. Isolated sacculi as well as purified… CONTINUE READING