Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers.

@article{Vajrabhaya1997MultilayerAM,
  title={Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers.},
  author={La-ongthong Vajrabhaya and Pongtip Sithisarn},
  journal={International endodontic journal},
  year={1997},
  volume={30 2},
  pages={
          141-4
        }
}
The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU-mu-1 (Mahidol University mouse cell line 1) were cultured in separate 96-well plates. Following incubation at 37 degrees C in 5% CO2 for 4 h in the presence of each sealer, cells were stained with 0.4% Sulphorhodamine-B, viable dye staining and the absorbance at 540 nm determined and calculated as cell viability. There was no… CONTINUE READING

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