Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos.

@article{Trivedi2018MultidimensionalQA,
  title={Multidimensional quantitative analysis of mRNA expression within intact vertebrate embryos.},
  author={Vikas Trivedi and Harry M T Choi and Scott E. Fraser and Niles A. Pierce},
  journal={Development},
  year={2018},
  volume={145 1}
}
For decades, in situ hybridization methods have been essential tools for studies of vertebrate development and disease, as they enable qualitative analyses of mRNA expression in an anatomical context. Quantitative mRNA analyses typically sacrifice the anatomy, relying on embryo microdissection, dissociation, cell sorting and/or homogenization. Here, we eliminate the trade-off between quantitation and anatomical context, using quantitative in situ hybridization chain reaction (qHCR) to perform… CONTINUE READING
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