Multicolor stimulated Raman scattering (SRS) microscopy.

  title={Multicolor stimulated Raman scattering (SRS) microscopy.},
  author={Fa-Ke Lu and Minbiao Ji and Dan Fu and Xiaohui Ni and Christian W. Freudiger and G. R. Holtom and Xiaoliang Sunney Xie},
  journal={Molecular physics},
  volume={110 15-16},
Stimulated Raman scattering (SRS) microscopy has opened up a wide range of biochemical imaging applications by probing a particular Raman-active molecule vibrational mode in the specimen. However, the original implementation with picosecond pulse excitation can only realize rapid chemical mapping with a single Raman band. Here we present a novel SRS microscopic technique using a grating-based pulse shaper for excitation and a grating-based spectrograph for detection to achieve simultaneous… CONTINUE READING