Multicolor fluorescent in situ mRNA hybridization (FISH) on whole mounts and sections.

@article{Lea2012MulticolorFI,
  title={Multicolor fluorescent in situ mRNA hybridization (FISH) on whole mounts and sections.},
  author={Robert William Lea and Boyan B Bonev and Eamon Dubaissi and Peter D. Vize and Nancy Papalopulu},
  journal={Methods in molecular biology},
  year={2012},
  volume={917},
  pages={431-44}
}
In situ hybridization involves the hybridization of an antisense RNA probe to an mRNA transcript and it is a powerful method for the characterization of gene expression in tissues, organs, or whole organisms. Performed as a whole mount (WISH), it allows the detection of mRNA transcripts in three dimensions, while combined with sectioning, either before or after hybridization, it provides gene expression information with cellular resolution. FISH relies on the fluorescence detection of probes… CONTINUE READING