Multicolor fluorescence in situ hybridization analysis of aneuploidy and diploidy frequencies in 225,846 sperm from 10 normal men.

@article{Martn1996MulticolorFI,
  title={Multicolor fluorescence in situ hybridization analysis of aneuploidy and diploidy frequencies in 225,846 sperm from 10 normal men.},
  author={R. Mart{\'i}n and E. Spriggs and A. Rademaker},
  journal={Biology of reproduction},
  year={1996},
  volume={54 2},
  pages={
          394-8
        }
}
Aneuploidy and diploidy frequencies for chromosomes 1, 12, X, and Y were assessed in 225,846 sperm from 10 normal men. Results from 5 of the men have previously been reported. Multicolor fluorescence in situ hybridization (FISH) was used to control for lack of probe hybridization and to distinguish diploidy from disomy. A minimum of 10,000 sperm per donor were evaluated for each chromosome. Sperm were considered disomic if two fluorescent signals were separated by a distance of a minimum of one… Expand
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Genetics of Human Sperm
  • Renée H. Martin
  • Biology, Medicine
  • Journal of Assisted Reproduction and Genetics
  • 2004
TLDR
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Findings confirm inter-chromosome differences in the frequencies of disomy and suggest that some apparently healthy men exhibit consistently elevated frequencies of specific sperm aneuplodies, which may be detected over as short a period as five years in a repeated-measures study. Expand
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Incidence of Chromosome 8, 10, X and Y Aneuploidies in Sperm Nucleus of Infertile Men Detected by FISH
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FISH analysis demonstrate a significantly increased frequency of aneuploidy for the sex chromosomes in sperm nuclei from infertile men, and it may be concluded that infertility is a risk factor for sex chromosome aneuPLoidy in spermuclei. Expand
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TLDR
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