Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets

@inproceedings{Zhao2016Multicolor4F,
  title={Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets},
  author={Teng Zhao and Sze Cheung Lau and Ying Wang and Yumian Su and Hao Wang and Aifang Cheng and Karl Herrup and Nancy Y Ip and Shengwang Du and M. M. T. Loy},
  booktitle={Scientific reports},
  year={2016}
}
We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of… CONTINUE READING