Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI

@article{Chinnasamy2005MulticistronicLV,
  title={Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI},
  author={Dhanalakshmi Chinnasamy and Michael D Milsom and James Shaffer and James Neuenfeldt and Aimen F Shaaban and G. P. Margison and Leslie J. Fairbairn and Nachimuthu Chinnasamy},
  journal={Virology Journal},
  year={2005},
  volume={3},
  pages={14 - 14}
}
A number of gene therapy applications would benefit from vectors capable of expressing multiple genes. In this study we explored the feasibility and efficiency of expressing two or three transgenes in HIV-1 based lentiviral vector. Bicistronic and tricistronic self-inactivating lentiviral vectors were constructed employing the internal ribosomal entry site (IRES) sequence of encephalomyocarditis virus (EMCV) and/or foot-and-mouth disease virus (FMDV) cleavage factor 2A. We employed enhanced… CONTINUE READING
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