Multichannel reverse transcription-polymerase chain reaction microdevice for rapid gene expression and biomarker analysis.

@article{Toriello2006MultichannelRT,
  title={Multichannel reverse transcription-polymerase chain reaction microdevice for rapid gene expression and biomarker analysis.},
  author={Nicholas Michael Toriello and Chung Nang Liu and Richard A Mathies},
  journal={Analytical chemistry},
  year={2006},
  volume={78 23},
  pages={
          7997-8003
        }
}
A microdevice is developed for RNA analysis that integrates one-step reverse transcription and 30 cycles of PCR (RT-PCR) amplification with capillary electrophoresis (CE) separation and fluorescence detection of the amplicons. The four-layer glass-PDMS-glass-glass hybrid microdevice integrates microvalves, on-chip heaters and temperature sensors, nanoliter reaction chambers (380 nL), and 5-cm-long CE separation channels. The direct integration of these processes results in attomolar detection… CONTINUE READING
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Integrated microfluidic bioprocessor for single-cell gene expression analysis.

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