Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis.

@article{Mary2013MulticentricEO,
  title={Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis.},
  author={C. F. Gleena Mary and Emmanuelle Chapey and Emmanuel Dutoit and Karine Guyot and Lilia Hasseine and Fakhri Jeddi and Jean Menotti and Carine Paraud and Christelle Pomares and Meja Rabodonirina and Ana{\"i}s Rieux and Francis Derouin},
  journal={Journal of clinical microbiology},
  year={2013},
  volume={51 8},
  pages={
          2556-63
        }
}
Cryptosporidium is a protozoan parasite responsible for gastroenteritis, especially in immunocompromised patients. Laboratory diagnosis of cryptosporidiosis relies on microscopy, antigen detection, and nucleic acid detection and analysis. Among the numerous molecular targets available, the 18S rRNA gene displays the best sensitivity and sequence variations between species and can be used for molecular typing assays. This paper presents a new real-time PCR assay for the detection and… CONTINUE READING
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