Multicenter validation study of two blockcycler- and one capillary-based real-time PCR methods for the detection of Salmonella in milk powder.

@article{Malorny2007MulticenterVS,
  title={Multicenter validation study of two blockcycler- and one capillary-based real-time PCR methods for the detection of Salmonella in milk powder.},
  author={Burkhard Malorny and Dietrich M{\"a}de and Paul Teufel and Cornelia Berghof-J{\"a}ger and Ingrid Huber and Annette Anderson and Reiner Helmuth},
  journal={International journal of food microbiology},
  year={2007},
  volume={117 2},
  pages={211-8}
}
A collaborative study including 13 German laboratories was conducted to evaluate the performance of two non-patented real-time PCR methods for the detection of Salmonella in milk powder targeting the ttrC/ttrA- or the invA gene. The enrichment procedure and sample DNA preparation method prior to the real-time PCR was the same for both systems and the identical DNA extraction samples were analysed. The traditional cultural method according to EN ISO 6579:2002 for the detection of Salmonella in… CONTINUE READING

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