MultiBac: multigene baculovirus-based eukaryotic protein complex production.

@article{Bieniossek2008MultiBacMB,
  title={MultiBac: multigene baculovirus-based eukaryotic protein complex production.},
  author={Christoph Bieniossek and Timothy J. Richmond and Imre Berger},
  journal={Current protocols in protein science},
  year={2008},
  volume={Chapter 5},
  pages={
          Unit 5.20
        }
}
Multiprotein complexes are an emerging focus in current biology, resulting in a demand for advanced heterologous expression systems. This unit provides protocols for the expression of eukaryotic multiprotein complexes using multigene expression vectors. Homologous and site-specific recombinases facilitate their assembly. Thus, modification of individual subunits for revised expression studies is achieved with comparative ease. The strategy outlined here employs the MultiBac baculoviral… CONTINUE READING
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