Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate.

@article{Iwamuro1999MultiubiquitinationOA,
  title={Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate.},
  author={S Iwamuro and Mari Saeki and Satoshi Kato},
  journal={Journal of biochemistry},
  year={1999},
  volume={126 1},
  pages={48-53}
}
During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyzed a clone showing a typical pattern of the ladder bands. This clone encoded a 188-amino acid polypeptide containing a putative transmembrane domain. A green fluorescent protein… CONTINUE READING