Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography.

@article{Wang1998MultisiteBO,
  title={Multi-site binding of fenoprofen to human serum albumin studied by a combined technique of microdialysis with high performance liquid chromatography.},
  author={He Wang and H Zou and Yu Kui Zhang},
  journal={Biomedical chromatography : BMC},
  year={1998},
  volume={12 1},
  pages={4-7}
}
A simple and fast method for the determination of the multi-site binding of fenoprofen (FP) to human serum albumin (HSA) has been developed by utilizing microdialysis sampling techniques combined with high performance liquid chromatography (HPLC). The drug and protein were mixed in different molar ratios in 0.067 Mol potassium phosphate buffer, pH 7.4, and incubated at 37 degrees C in a water-bath. Then the microdialysis probe was put in the FP-HSA solution and sampled at the perfusion rate of… CONTINUE READING