Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing

@inproceedings{Oakes2016MultireporterSF,
  title={Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing},
  author={Benjamin L. Oakes and Danny F. Xia and Elizabeth F. Rowland and Denise J. Xu and Irina Ankoudinova and Jennifer S. Borchardt and Lei Zhang and Patrick Li and Jeffrey C. Miller and Edward J. Rebar and Marcus B. Noyes},
  booktitle={Nature communications},
  year={2016}
}
Engineered nucleases have transformed biological research and offer great therapeutic potential by enabling the straightforward modification of desired genomic sequences. While many nuclease platforms have proven functional, all can produce unanticipated off-target lesions and have difficulty discriminating between homologous sequences, limiting their therapeutic application. Here we describe a multi-reporter selection system that allows the screening of large protein libraries to uncover… CONTINUE READING

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