Multi-protein complexes in eukaryotic gene transcription

  title={Multi-protein complexes in eukaryotic gene transcription},
  author={Ernest Martinez},
  journal={Plant Molecular Biology},
  • E. Martinez
  • Published 1 December 2002
  • Biology
  • Plant Molecular Biology
Specific transcription initiation by RNA polymerase II at eukaryotic protein-coding genes involves the cooperative assembly at the core promoter of more than 40 distinct proteins – with a total mass of over 2 MDa – including RNA polymerase II itself and general/basal transcription initiation factors, to form a stable pre-initiation complex (PIC). In vivo, PIC assembly is a major point of regulation by sequence-specific transcription regulators (activators and repressors) and is hindered by the… 
Structure and Basal Transcription Complex of RNA Polymerase II Core Promoters in the Mammalian Genome: An Overview
TFIID plays a central role in recognizing and binding specific core promoter elements to support creating an environment that facilitates transcription initiation, and is associated with the majority of protein-coding genes within the mammalian genome.
The Saccharomyces cerevisiae Paf1 transcription elongation complex is connected to chromatin modification through the multifunctional Rtf1 subunit and the inositol polyphosphate signaling pathway
Strong genetic interactions between Arg82, Paf1, and mutations in the SWI/SNF and INO80 chromatin remodeling complexes were uncovered and it was demonstrated that the expression of several target genes was strongly impaired by mutations in these factors.
Mechanism of transcription initiation by the yeast mitochondrial RNA polymerase.
Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.
The results provide for the first time a general picture of GTF function during the RNAPII transcription reaction in live mammalian cells and show that TFIIF and Rpb7 are involved in both early and late transcriptional stages.
TcoF-DB: dragon database for human transcription co-factors and transcription factor interacting proteins
The Dragon Database for Human Transcription Co-Factors and Transcription Factor Interacting Proteins (TcoF-DB) is developed, comprised of a collection of human TFs and the TcoFs with which they interact.
Mechanisms of Core Promoter Sequence-dependent RNA Polymerase II Transcription
Analysis of activated transcription by Gal4-fusion activators and the beta-Actin gene (ACTB) promoter upstream activating sequences further demonstrates that preferential communication between activator and core promoters contributes to the gene expression regulation.
Identification and characterization of novel protein-protein interactions with the basal transcription factor, TATA-binding protein
The studies, in agreement with previous published data suggest that TBP interacts with many classes of regulatory proteins, including transcriptional activators, repressors, and individual components of the transcriptional co-regulatory complexes.
Involvement of general transcriptional factors in the regulation of transcription of the hsp70 gene in vivo
It was shown that, before heat induction, nearly all general transcriptional factors are present on the hsp70 gene promoter, however, after heat exposure, the amount of TBP, TAF, TFIIB, and TFIIF decreases, whereas the amounts of RNA polymerase II,TFIIH, GCN5, TRRAP, and Med13 increases.
STAGA Recruits Mediator to the MYC Oncoprotein To Stimulate Transcription and Cell Proliferation
A novel STAF65γ-dependent function of STAGA-type complexes in cell proliferation and transcription activation by MYC postloading of TFIID and RNA polymerase II that involves direct recruitment of core Mediator is suggested.


Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery
  • M. Hampsey
  • Biology
    Microbiology and Molecular Biology Reviews
  • 1998
This review focuses on the global effectors of RNA polymerase II transcription in yeast, including the general transcription factors, the coactivators, and the general repressors.
An RNA polymerase II holoenzyme responsive to activators
It is proposed that the holoenzyme is a form of RNA polymerase II readily recruited to promoters in vivo, a multi-subunit complex containing roughly equimolar amounts of RNA Polymerase II, a subset of general transcription factors, and SRB regulatory proteins.
Eukaryotic transcription factor-DNA complexes.
X-ray crystallography and nuclear magnetic resonance spectroscopy have been used to study complexes of general transcription factors and transcriptional activators with their specific DNA targets and provided important structural insights into transcription initiation by polymerase II and the more general problem of DNA sequence recognition.
Biochemistry and structural biology of transcription factor IID (TFIID).
This work has shown that TFIID, a 700-kD complex composed of the TATA box binding protein (TBP) and a set of phylogenetically conserved, polymerase-specific TBP-associated factors or TAFIIS, play critical regulatory roles in eukaryotic gene expression.
TBP-associated factors (TAFIIs): multiple, selective transcriptional mediators in common complexes.
  • M. Green
  • Biology
    Trends in biochemical sciences
  • 2000
Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II.
Results indicate that TBP-free RNA polymerase II mediated transcription may be able to occur in mammalian cells and that multiple preinitiation complexes may play an important role in regulating gene expression.
Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter.
The development of a human cell line expressing an epitope-tagged TBP and the immunopurification of a native, high-molecular-weight form of TFIID that supports transcriptional stimulation by several different classes of activation domains are reported.
Core promoter-specific function of a mutant transcription factor TFIID defective in TATA-box binding.
The results show that a functional initiator element is needed to bypass the requirement for an active TATA DNA-binding surface in TFIID and imply that gene-specific transcription can be achieved by modulating distinct core promoter-specific TFIid functions--e.g., TBP-TATA versus TAF-initiator interactions.
A dynamic model for PC4 coactivator function in RNA polymerase II transcription.
PC4 coactivator activity is realized in a stepwise series of events reminiscent of prokaryotic activation pathways involving conversion of inactive RNA polymerase-promoter complexes to an initiation-competent state.