Multi-photon microscopy: seeing more by imaging less.

@article{White2002MultiphotonMS,
  title={Multi-photon microscopy: seeing more by imaging less.},
  author={Nick S. White and Rachel J. Errington},
  journal={BioTechniques},
  year={2002},
  volume={33 2},
  pages={
          298-300, 302, 304-5
        }
}
Since the earliest microscopes of Leuwenhoek and Hooke in the 17th century, optical designers have strived to maximize a key performance characteristic of each new instrument model—that of contrast. Put simply, contrast is how well we can discern useful information about a microscopic specimen from unwanted background and noise. Resolution (the ability to accurately measure features) and sensitivity (the ability to detect features) are intimately related to contrast. The Rayleigh criterion for… CONTINUE READING
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