Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture.

@article{Okkelman2017MultiParametricIO,
  title={Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture.},
  author={Irina A. Okkelman and Tara Foley and Dmitri B. Papkovsky and Ruslan I Dmitriev},
  journal={Advances in experimental medicine and biology},
  year={2017},
  volume={1035},
  pages={85-103}
}
Dynamics of oxygenation of tissue and stem cell niches are important for understanding physiological function of the intestine in normal and diseased states. Only a few techniques allow live visualization of tissue hypoxia at cellular level and in three dimensions. We describe an optimized protocol, which uses cell-penetrating O2-sensitive probe, Pt-Glc and… CONTINUE READING