Mucopolysaccharides and mucoproteins of calf scapula

  title={Mucopolysaccharides and mucoproteins of calf scapula},
  author={Arthur Lindenbaum and Klaus E. Kuettner},
  journal={Calcified Tissue Research},
In the ossifying calf scapula the sharp division between cartilage and bone was found to allow collection of nearly unlimited quantities of both cartilage and bone in any desired stage of the transformation process. The mucopolysaccharides (MPS) of all zones consist mainly of galactosaminoglycans of the chondroitin sulfate A and C type. The highest concentration of MPS and mucoproteins (MP) was found in the 2 mm-wide cartilage zone adjacent to the bone. Significant differences among cartilage… 
Lysozyme in preosseous cartilage
A significant rise in lysozyme concentration was found toward the ossification front in scapular cartilage as was a sharp drop in new bone as the animal matured and the growth rate of bone decreased.
Resistant proteoglycans in epiphyseal plate cartilage. Variations in their distribution in relationship to age and species
The localizations of resistant proteoglycans (RPGs) in the epiphyseal plates of rats, dogs, and humans are similar, but calcified cartilage RPGs stain more intensely with toluidine blue and may represent a different type of RPGs.
A zonal analysis of inorganic and organic constituents of the epiphysis during endochondral calcification
  • R. Wuthier
  • Biology, Medicine
    Calcified Tissue Research
  • 2005
A dissection procedure has been devised to permit zonal analysis of the epiphyseal plate of fetal calf leg bones for their content of electrolyte and organic constituents, as well as for density, ash and moisture, which showed that as ash content and density increased, water content decreased.
The influence of lysozyme on the appearance of epiphyseal cartilage in organ culture
The concept that lysozyme may have a functional role in either osteogenesis, chondrogenesis, or calcification is supported, as well as the fact that the distribution of these changes appears to be the same as that of the gross darkening effect.
Microchemical studies on acid glycosaminoglycans of the epiphyseal zones during endochondral calcification
Data obtained by the CPC microfractionation procedure indicated similar distribution according to molecular weight and/or charge density for extractable and unextractable chondroitin sulphate in nasal septum cartilage and in resting and columnar zones of epiphyseal growth plate, suggesting a stronger binding of proteoglycans within the tissue.
Composition and calcium binding of proteinpolysaccharides of calf nasal septum and scapula
The data obtained from this study do not indicate sufficient differences between the chemical composition and calcium affinity of proteinpolysaccharides from “resting” and “ossifying” scapular cartilage to account for the calcium uptake in the “passive” zone.
Dynamic changes in acid mucopolysaccharides during mineralization of the mandibular condylar cartilage
The results indicate that AMPS are synthesized and secreted mainly by hypertrophic chondrocytes in the premineralizing zone and within the matrix at the mineralization front the AMPS complexes are apparently degraded by lysosomal enzymes to yield a highly anionic fraction which is maintained in the matrix.
A review of recent advances in the chemistry of calcifying cartilage and bone matrix
Bone contains glycoproteins, acidic lipids and peptides which could have a potential function in nucleation, ion-transport and control in calcification processes, and cartilage contains similar lipids are present; glycoproteinins may be inferred from the sialic acid content; the presence of peptides is not yet known.
Die Calcifikation bei der Knochenbildung
  • K. Münzenberg
  • Chemistry
    Archiv für orthopädische und Unfall-Chirurgie, mit besonderer Berücksichtigung der Frakturenlehre und der orthopädisch-chirurgischen Technik
  • 2004
SummaryThe calcification during formation of bone is a biological process in which cells play a central role. But also it is dependent on physicochemical conditions and crystallographic
The observation, which shows that lysozyme, when added to cartilage, accumulates in specific anatomic sites suggests that it chemically interacts with specific components of cartilage matrix, presumably with negatively charged macromolecules.


The acid mucopolysaccharides of connective tissue.
Protein- Polysaccharide Loss during Endochondral Ossification: Immunochemical Evidence
Fluorescein labeled antibodies to protein- polysaccharides from rat and calf cartilage were used for the histochemical localization of protein- poly saccharide in epiphyseal cartilage. There was less
Comparison of Bovine Bone Sialoprotein and Serum Orosomucoid
It was considered to be of some interest, therefore, to compare these two substances under similar conditions of isolation and analysis.
[Physiology and biochemistry of bone formation].
  • H. Fleisch
  • Chemistry, Medicine
    Klinische Wochenschrift
  • 1966
Der Aufbau und die Struktur der drei Hauptbestandteile des Knochens, die Zellen, das organische Stroma und das Mineral, wurde besprochen.
Heparinic Acids: Determination of Equivalent Weights and Sulfate to Carboxyl Ratios
By means of an automatic titration apparatus, a polyelectrolyte of biological origin, heparin, from two animal sources, was found to have an average equivalent weight of 170.0, a sulfate content of