Mucin biosynthesis: molecular cloning and expression of bovine lung mucin core 2 N-acetylglucosaminyltransferase cDNA.

@article{Li1998MucinBM,
  title={Mucin biosynthesis: molecular cloning and expression of bovine lung mucin core 2 N-acetylglucosaminyltransferase cDNA.},
  author={C M Li and Kenneth B. Adler and P W Cheng},
  journal={American journal of respiratory cell and molecular biology},
  year={1998},
  volume={18 3},
  pages={
          343-52
        }
}
  • C. Li, K. Adler, P. Cheng
  • Published 1 March 1998
  • Biology
  • American journal of respiratory cell and molecular biology
A cDNA clone containing a 2,150-bp insert was isolated from a bovine lung lambdagt10 cDNA library by cross-species hybridization using a DNA probe generated by polymerase chain reaction (PCR) employing a human cDNA that encodes mucin core 2 beta6-N-acetylglucosaminyltransferase (hC2TF) as the template. The bovine cDNA (bcDNA) insert was devoid of 220 bp of the 5' portion of the C2TF open reading frame (ORF), as predicted from the human counterpart. Southern blotting analysis suggested that the… 
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Mucin biosynthesis: molecular cloning and expression of mouse mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase.
TLDR
Antibodies generated against mC2GnT-M identified this enzyme in the goblet cells and other mucus cells/glands, which supports the important role of this enzymes in the protective functions of secreted mucins.
Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue.
TLDR
The results suggest that BHV-4 acquired its C2GnT-M gene from the bovine gene, and the mechanism of the viral acquisition and the roles of the C2gnT -M gene in the survival and pathogenesis of this virus remain to be elucidated.
Expression of Core 2 β-1,6-N-Acetylglucosaminyltransferase in a Human Pancreatic Cancer Cell Line Results in Altered Expression of MUC1 Tumor-associated Epitopes*
TLDR
The stable transfection of a human pancreatic adenocarcinoma cell line with the cDNA for mucin core 2 GlcNAc-transferase (C2GnT), which creates the core 2 β-1,6 branch in mucin-type glycans, suggests that C2GNT could play a regulatory role in the expression of certain tumor-associated epitopes.
Identification of Disulfide Bonds among the Nine Core 2 N-Acetylglucosaminyltransferase-M Cysteines Conserved in the Mucin β6-N-Acetylglucosaminyltransferase Family*
TLDR
Molecular modeling using disulfide bond assignments and the fold recognition/threading method to search the Protein Data Bank found a match with aspartate aminotransferase structure, different from the two major protein folds proposed for glycosyltransferases.
Mucin biosynthesis: upregulation of core 2 beta 1,6 N-acetylglucosaminyltransferase by retinoic acid and Th2 cytokines in a human airway epithelial cell line.
TLDR
The results suggest that IL-4/13 T helper 2 cytokines and RA can alter the activity of enzymes that synthesize branching mucin glycan beta1,6-branched structures in airway epithelial cells, potentially leading to altered mucin carbohydrate structure and properties.
Soluble human core 2 beta6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity.
Site directed processing: role of amino acid sequences and glycosylation of acceptor glycopeptides in the assembly of extended mucin type O-glycan core 2.
Biosynthesis and function of β 1,6 branched mucin-type glycans
The contribution of carbohydrate structure to biomolecular, cellular, and organismal function is well-established, but has not yet received the attention it deserves, perhaps due to the complexity of…
Cell type-specific activation of the cytomegalovirus promoter by dimethylsulfoxide and 5-aza-2'-deoxycytidine.
Core 2 β6-N- Acetylglucosaminyltransferase-I and -III
TLDR
Poly-N-acetyllactosaminyl chain can be extended from the GlcNAcβ1,6- linkage synthesized in core 2, and can be further modified by α3 fucosyltransferase, forming sialyl Lex termini.
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    Proceedings of the National Academy of Sciences of the United States of America
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