Movement of motor and cargo along cilia

  title={Movement of motor and cargo along cilia},
  author={Jose T. Orozco and Karen P. Wedaman and Dawn Signor and Heather M. Brown and Lesilee S. Rose and Jonathan M. Scholey},
Intraflagellar transport (IFT) is important in the formation and maintenance of many cilia, such as the motile cilia that drive the swimming of cells and embryos, the nodal cilia that generate left-right asymmetry in vertebrate embryos, and the sensory cilia that detect sensory stimuli in some animals. The heterotrimeric kinesin-II motor protein drives the anterograde transport of macromolecular complexes, called rafts, along microtubule tracks from the base of the cilium to its distal tip… Expand
Intraflagellar transport motors in cilia: moving along the cell's antenna
  • J. Scholey
  • Biology, Medicine
  • The Journal of cell biology
  • 2008
My perspective on IFT as a model system for studying motor-driven cargo transport is presented and it is hypothesized that several accessory kinesins confer cilia-specific functions by augmenting the action of the two core IFT motors, kinesin-2 and dynein 1b, which assemble the cilium foundation. Expand
Two anterograde intraflagellar transport motors cooperate to build sensory cilia on C. elegans neurons
It is shown that two anterograde IFT motor holoenzymes, kinesin-II and Osm-3–kinesin, cooperate in a surprising way to control two pathways of IFT that build distinct parts of cilia. Expand
Analysis of intraflagellar transport in C. elegans sensory cilia.
How the tools of genetics, cell biology, electron microscopy, and biochemistry are being used to dissect the composition and mechanism of action of IFT motors and IFT particles in C. elegans is summarized. Expand
Intraflagellar transport: mechanisms of motor action, cooperation, and cargo delivery
Intraflagellar transport (IFT) is a form of motor‐dependent cargo transport that is essential for the assembly, maintenance, and length control of cilia, which play critical roles in motility,Expand
Measuring rates of intraflagellar transport along Caenorhabditis elegans sensory cilia using fluorescence microscopy.
Transport assays are described that allow measurements of the rates of movement of specific, fluorescently tagged, functional components of the IFT machinery, including motors, IFT particle subunits, and putative cargo, and the identification of IFT-dynein subunits and ciliary tubulin isotypes as likely cargo proteins of kinesin-2-driven anterograde IFT. Expand
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors
Using competitive in vitro MT gliding assays, it is shown that purified kinesin-II and OSM-3 cooperate to generate movement similar to that seen along the cilium in the absence of any additional regulatory factors. Expand
Functional coordination of intraflagellar transport motors
Findings implicate BBS ciliary disease proteins and an OSM-3 kinesin activator in the formation of two IFT pathways that build functional cilia. Expand
Functional differentiation of cooperating kinesin-2 motors orchestrates cargo import and transport in C. elegans cilia
Intracellular transport depends on cooperation between distinct motor proteins. Two anterograde intraflagellar transport (IFT) motors, heterotrimeric kinesin-II and homodimeric OSM-3, cooperate toExpand
Kinesin-2 motors transport IFT-particles, dyneins and tubulin subunits to the tips of Caenorhabditis elegans sensory cilia: Relevance to vision research?
The work on kinesin-2 motors, intraflagellar transport (IFT) and cilium biogenesis in C. elegans sensory cilia is reviewed, and its possible relevance to current research on vertebrate photoreceptor cilia assembly and function is commented on. Expand
Intraflagellar transport motors in Caenorhabditis elegans neurons.
The protein machinery of IFT in C. elegans is described and analysed using time-lapse fluorescence microscopy of green fluorescent protein-fusion proteins in concert with ciliary mutants. Expand


Novel heterotrimeric kinesin-related protein purified from sea urchin eggs
It is reported here that kinesin-related genes encode the 85K and 95K subunits, and that the protein can be immunoprecipitated from cytosol as a trimeric complex using an 85K monoclonal antibody. Expand