Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion Limit

@article{Wolf1989MovementPO,
  title={Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion Limit},
  author={Shmuel Wolf and William J Lucas and Carl Michael Deom and Roger N. Beachy},
  journal={Science},
  year={1989},
  volume={246},
  pages={377 - 379}
}
The function of the 30-kilodalton movement protein (MP) of tobacco mosaic virus is to facilitate cell-to-cell movement of viral progeny in an infected plant. A novel method for delivering non-plasmalemma-permeable fluorescent probes to the cytosol of spongy mesophyll cells of tobacco leaves was used to study plasmodesmatal size exclusion limits in transgenic plants that express the MP gene. Movement of fluorescein isothiocyanate-labeled dextran (F-dextran) with an average molecular mass of 9400… 
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TLDR
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Plasmodesmatal Function 1s Probed Using Transgenic Tobacco Plants That Express a Virus Movement Protein
TLDR
Surprisingly, at 32OC, movement of the F-dextrans was inhibited in transgenic plants expressing the wild-type MP gene; however, the inhibition was transient and was no longer detected after 48 hours at this elevated temperature, providing experimental evidence that callose deposition is involved in regulating the molecular size-exclusion limit of plasmodesmata in plants.
An extraction method for tobacco mosaic virus movement protein localizing in plasmodesmata
TLDR
A method for differential extraction of MP from isolated cell walls of transgenic tobacco leaves expressing MP or MP tagged with green-fluorescent protein is developed, which might be a powerful tool for investigating MP-interacting proteins in branched PD.
Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein.
TLDR
Surprisingly, at 32 degrees C, movement of the F-dextrans was inhibited in transgenic plants expressing the wild-type MP gene; however, the inhibition was transient and was no longer detected after 48 hours at this elevated temperature, providing experimental evidence that callose deposition is involved in regulating the molecular size-exclusion limit of plasmodesmata in plants.
Effect of virus infection on symplastic transport of fluorescent tracers in Nicotiana clevelandii leaf epidermis
TLDR
The results imply that intercellular movement of these viruses does not involve a long-lasting increase in the plasmodesmatal molecular size exclusion limit.
Localization of the Tobacco Mosaic Virus Movement Protein in Transgenic Tobacco Plants
TLDR
The structure and function of plasmodesmata, particularly those aspects related to virus movement, are examined as a functlon of leaf development in young leaves of 30-kD TMV MP transgenic and vector control plants.
Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants.
TLDR
The structure and function of plasmodesmata, particularly those aspects related to virus movement, are examined as a function of leaf development in young leaves of 30-kD TMV MP transgenic and vector control plants.
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