Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid.

@article{Fissore1989MouseZD,
  title={Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid.},
  author={Rafael A. Fissore and Katharine V. Jackson and Ann A. Kiessling},
  journal={Biology of reproduction},
  year={1989},
  volume={41 5},
  pages={835-41}
}
Development of zygotes from a hybrid-inbred (B6D2F1) and two random-bred (CD1 and CF1) strains of mice were compared after culture in several modifications of a simple, chemically defined medium based on Earle's Balanced Salts Solution. When cultured without the addition of protein or the chelating agent, ethylenediaminetetraacetic acid (EDTA), none of the zygotes reached the blastocyst stage. The addition of EDTA or protein significantly improved embryo development to blastocysts (p less than… CONTINUE READING

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