Mouse sperm chromatin proteins: quantitative isolation and partial characterization.

  title={Mouse sperm chromatin proteins: quantitative isolation and partial characterization.},
  author={Rod Balhorn and Barton L Gledhill and Andrew J. Wyrobek},
  volume={16 18},
Conditions are described that permit the quantitative extraction of chromatin proteins from the epididymal sperm of the mouse. These proteins have been isolated free of contaminating tail proteins following removal of the tails with cetyltrimethylammonium bromide (CTAB). Without this treatment, numerous acid-soluble tail proteins coextract with the nuclear proteins isolated from partially purified heads. The proteins isolated in this manner do not require prior modification with iodoacetamide… CONTINUE READING

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