Mouse liver cell culture

  title={Mouse liver cell culture},
  author={James E Klaunig and Peter J. Goldblatt and David E. Hinton and Michael M. Lipsky and Benjamin F. Trump},
  journal={In Vitro},
Mouse hepatocytes in primary culture were characterized. Hepatocytes were isolated by the two-step hepatic portal vein perfusion method described previously. An optimal cell attachment of 43% was noted after 2 h incubation in 10% fetal bovine serum. Minimal attachment (lees than 7%) occurred in serumless medium. Serum concentrations above 10% and attachment durations greater than 2 h resulted in no increased attachment of viable cells. Nonviable cells, however progressively attached when both… CONTINUE READING
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