Mouse embryonic stem cell adherent cell differentiation and cytotoxicity (ACDC) assay.

@article{Barrier2011MouseES,
  title={Mouse embryonic stem cell adherent cell differentiation and cytotoxicity (ACDC) assay.},
  author={M. Barrier and Susan C. Jeffay and Harriette P Nichols and Kelly J. Chandler and Maria R Hoopes and Kimberly Slentz-Kesler and E. Sidney Hunter},
  journal={Reproductive toxicology},
  year={2011},
  volume={31 4},
  pages={
          383-91
        }
}
An adherent cell differentiation and cytotoxicity (ACDC) assay was developed using pluripotent J1 mouse embryonic stem cells (mESCs). Adherent mESCs were used to evaluate chemical-induced effects on both stem cell viability and differentiation using an in-cell western technique after a 9-day culture. DRAQ5/Sapphire700 stains were used to quantify cell number. Myosin heavy chain protein was used as a marker of cardiomyocyte differentiation and was corrected for cell number, thereby separating… CONTINUE READING

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