Mouse PeP: A novel peroxisomal protein linked to myoblast differentiation and development

@article{FerrerMartnez2002MousePA,
  title={Mouse PeP: A novel peroxisomal protein linked to myoblast differentiation and development},
  author={Andreu Ferrer-Mart{\'i}nez and Pilar Ruiz-Lozano and Kenneth R Chien},
  journal={Developmental Dynamics},
  year={2002},
  volume={224}
}
The identification of several peroxisomal proteins in the past decade has deepened our understanding of the biology of peroxisomes and their involvement in human disorders. We report the cloning and expression pattern during the mouse development of a cDNA encoding a novel protein, named PeP, and show that its product is imported specifically to the peroxisome matrix in a variety of cell types. We also demonstrate that PeP is imported to the organelle through the PEX5 receptor pathway, which… Expand
The expression of peroxisomal protein transcripts increased by retinoic acid during neural differentiation.
TLDR
The results revealed that expression of PEP transcripts was markedly increased after the RA treatment at embryoid body and neural stages, which concluded that PEP might be involved in the early process of neurogenesis, which needs further verification. Expand
Mouse Peroxisomal Protein cDNA Cloning and Characterization of its Intraclleular Localization
TLDR
To investigate the intracellular localization of PEP protein linked to EGFP marker, the constructed plasmid was used for transfection into the chinese hamster ovary (CHO) cells and data showed that PEP is a peroxisomal protein. Expand
Sorting analysis of mouse peroxisomal protein by in vitro studies
TLDR
The recently cloned peroxisomal matrix protein (PeP), contains two hydrophobic domains at 12-31 and 152169 amino acid residues and a fibronectin type III (FnIII) domain between residues 31-114, and site-directed mutagenesis was performed to delete these domains separately. Expand
Analysis of PEP Expression Pattern during Cardiogenesis of Mouse Embryonic Stem Cells
TLDR
Results demonstrated that expression of PEP transcripts was markedly increased at matured car‑ diomyocyte, implying that PEP might be involved in late cardiac cell differentiation which needs further verification implying a possible role in this process. Expand
Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
TLDR
The results showed that FNDC5 gene in Meishan pigs contains five transcripts, all of which can be translated into functional intact irisin proteins, which provide valuable information related to the study on F NDC5 functions and future development of novel treatment for obesity. Expand
Biochemistry of mammalian peroxisomes revisited.
TLDR
The identification and characterization of yeast mutants defective either in the biogenesis of peroxisomes or in one of its metabolic functions, notably fatty acid beta-oxidation, combined with the recognition of a group of genetic diseases in man, wherein these processes are also defective, have provided new insights in all aspects of perxisomes. Expand
CYTOSOLIC LOCALIZATION OF MOUSE PEROXISOMAL PROTEIN/ΔSKI FUSED WITH ENHANCED GREEN FLUORESCENT PROTEIN INTO CHINESE HAMSTER OVARY-K1 AND P19 CELLS
TLDR
Amino acid alignment analysis of deduced amino acid residues revealed a tripeptide (SKI) at the carboxy terminus of peroxisomal protein cDNA, which strongly suggest that SKI, which is located at the C- terminus, is required for sorting this protein. Expand
Characterization of fibronectin type III domain-containing protein 5 (FNDC5) gene in chickens: Cloning, tissue expression, and regulation of its expression in the muscle by fasting and cold exposure.
TLDR
The data suggest that FNDC5/irisin is more than a 'myokine' and may be related to the development/functions of many tissues (e.g. muscle, brain, fat), as well as metabolic status of chickens. Expand
Identification, Cloning, and Functional Analysis of the TATA-Less Mouse FNDC5 Promoter During Neural Differentiation
TLDR
Stage dependent expression of FNDC5 is affected by neural induction method used for neural differentiation, and data implicated that both exon 1 and intron 1 of the gene are included in the core promoter. Expand
INTRACELLULAR LOCALIZATION OF FLAG-PEROXISOMAL PROTEIN IN CHINESE HAMSTER OVARY (CHO) CELLS
TLDR
The aim of this study was to sub-clone the peroxisomal protein (PEP) cDNA into a mammalian expression vector leading to the formation of a chimeric PEP-cDNA containing the FLAG epitope. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 50 REFERENCES
Identification and characterization of the human peroxin PEX3.
The biogenesis of peroxisomes requires the interaction of several peroxins, encoded by PEX genes and is well conserved between yeast and humans. We have cloned the human cDNA of PEX3 based on itsExpand
Peroxisome through cell differentiation and neoplasia
TLDR
Peroxisomes are more and more involved in oxidative metabolic pathways as intestinal epithelial cells differentiate and a relationship is found between the specific activities of some peroxisomal enzymes and the histological tumour grades. Expand
Components involved in peroxisome import, biogenesis, proliferation, turnover, and movement.
TLDR
Important new insights are revealed regarding the mechanism of protein translocation across the peroxisomal membrane, the conservation of PEX genes through evolution, the role of peroxins in fatal human peroxISomal disorders, and the biogenesis of the organelle. Expand
Developmental and pathological expression of peroxisomal enzymes: their relationship of d-bifunctional protein deficiency and Zellweger syndrome
TLDR
It is shown that the peroxisomal enzymes may be closely related to neuronal maturation and gliogenesis in human brain and to disturbance of neuronal migration as seen in Zellweger syndrome. Expand
The four murine peroxisomal ABC-transporter genes differ in constitutive, inducible and developmental expression.
TLDR
Different expression patterns could explain why beta-oxidation is defective in X-ALD, although ALDRP and PMP70 can replace ALDP functionally in fibroblasts, and the use of ALDP-deficient mice as a model in pharmacological gene therapy studies. Expand
An oligomeric protein is imported into peroxisomes in vivo
TLDR
It is shown that the peroxisomal targeting sequences SKL or AKL, with or without a spacer of nine glycines (G9), are sufficient to target chloramphenicol acetyltransferase (CAT) toperoxisomes of Saccharomyces cerevisiae in vivo. Expand
Localization of mRNAs for adrenoleukodystrophy and the 70 kDa peroxisomal (PMP70) proteins in the rat brain during post‐natal development
TLDR
Using in situ hybridization histochemistry in rat brain, it is demonstrated that ALD and PMP70 mRNAs have different spatial and temporal expression during postnatal development, suggesting that AlD andPMP70 proteins are unlikely to function as exclusive and obligatory partners in the brain. Expand
An impaired peroxisomal targeting sequence leading to an unusual bicompartmental distribution of cytosolic epoxide hydrolase
TLDR
The carboxy terminal region of rat liver cEH is analyzed by means of cDNA cloning to define the structure of its possible peroxisomal targeting sequence (PTS), and the deduced amino acid sequence displays a terminal tripeptide Ser‐Lys‐Ile which is highly homologous to the PTS found in other peroxISomal enzymes. Expand
Peroxisomal beta-oxidation enzyme gene expression in the developing mouse brain
TLDR
Comparison of these developmental profiles with that obtained for ceramide galactosyltransferase showed that the set-up of the very-long-chain fatty acids beta-oxidation system is independent of the myelinating signal in the central nervous system. Expand
A novel, cleavable peroxisomal targeting signal at the amino‐terminus of the rat 3‐ketoacyl‐CoA thiolase.
TLDR
It is concluded that a novel PTS is identified that functions at amino‐terminal or internal locations and is distinct from the C-terminal PTS, which implies the existence of two different routes for targeting proteins into the peroxisomal matrix. Expand
...
1
2
3
4
5
...