Motility and substratum adhesion of Dictyostelium wild-type and cytoskeletal mutant cells: a study by RICM/bright-field double-view image analysis.

@article{Weber1995MotilityAS,
  title={Motility and substratum adhesion of Dictyostelium wild-type and cytoskeletal mutant cells: a study by RICM/bright-field double-view image analysis.},
  author={Igor Weber and E Wallraff and Richard Albrecht and G{\"u}nther Gerisch},
  journal={Journal of cell science},
  year={1995},
  volume={108 ( Pt 4)},
  pages={1519-30}
}
To investigate the dynamics of cell-substratum adhesion during locomotion, a double-view optical technique and computer-assisted image analysis has been developed which combines reflection interference contrast microscopy (RICM) with bright-field imaging. The simultaneous recording of cell-substratum contact and cell body contour has been applied to aggregation-competent cells of Dictyostelium discoideum. These cells are distinguished from cells at earlier stages of development by small areas… CONTINUE READING
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