Mother-child double incompatibility at vWA and D5S818 loci in paternity testing

  title={Mother-child double incompatibility at vWA and D5S818 loci in paternity testing},
  author={Venkanna Narkuti and Ravi N Vellanki and K P C Gandhi and Lakshmi Narasu Mangamoori},
  booktitle={Clinical chemistry and laboratory medicine},
Abstract Background: In a paternity dispute case, 17 autosomal short tandem repeats (STR) were examined and signified a possible paternal mismatch at vWA locus and a maternal mismatch at D5S818 locus in the child under investigation. Methods: Seventeen autosomal, 17 Y-chromosome and six X-chromosome repeat loci were used in parentage analysis. The mutated vWA and D5S818 alleles were amplified, cloned and sequenced to analyze the repeat structure. Results: The vWA locus genotype in the mother… 

Figures and Tables from this paper

Risk analysis of duo parentage testing with limited STR loci.
Whether the Goldeneye 20A system (containing 19 short tandem repeats) can avert the shortage of duo parentage tests is evaluated and the mutation and exclusion rates detected accorded with the corresponding rates identified in the Identifiler system.
Multi step microsatellite mutations leading to mother-child double variance--a case of non-exclusion parentage.
Investigation of Different Ways in Which the CODIS 7.0 May be Used in Mass Disaster Identification
A novel strategy is suggested to overcome limitations and increase efficiency in conducting identification of mass disaster victims by relating family relatives to a pedigree tree.
Haplotype-assisted characterization of germline mutations at short tandem repeat loci
In this study, 98 families with 101 mutations were analyzed in depth in which a mutation had been observed at one of the four loci D3S1358, FGA, ACTBP2, and VWA and it was possible to identify the mutated structure and/or mutation event in the vast majority of cases.
Paternal exclusion: allele sharing in microsatellite testing
Investigations on inconclusive paternity due to atypical allele sharing in autosomal microsatellites were resolved with X- and Y-chromosome STR analyses confirming the case as non-paternity.


Genetic analysis of 18 X-linked short tandem repeat markers in Korean population.
Short tandem repeat-based identification of individuals and parents.
Estimation of short tandem repeat (STR) multilocus genotype frequencies for the identification of individuals and estimation of allele frequencies for parentage assignment both depend on (a) testing
Application of forensic DNA testing in the legal system.
DNA technology has taken an irreplaceable position in the field of the forensic sciences. Since 1985, when Peter Gill and Alex Jeffreys first applied DNA technology to forensic problems, to the
Double incompatibility at human alpha fibrinogen and penta E loci in paternity testing.
The use of the approach, based on two amplification and detection formats and on the sequence analysis, confirmed the observed meiotic paternal mutations.